Cloned (Comment) | Organism |
---|---|
expression in Saccharomyces cerevisiae | Saussurea medusa |
gene SmDFR, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative real-time PCR expression analysis, functional expression in Saccharomyces cerevisiae strain INV Sc1 | Saussurea medusa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38600 | - |
x * 38600, SDS-PAGE | Saussurea medusa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Saussurea medusa | the enzyme catalyzes the reduction of dihydroflavonols to leucoanthocyanins. But SmDFR also possesses flavanone 4-reductase (FNR) activity and can catalyze the conversion of eridictyol to luteoforol, EC 1.1.1.234 | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saussurea medusa | A5Z0G1 | gene SmDFR | - |
Saussurea medusa | A5Z0G1 | putative | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
callus | a red callus line | Saussurea medusa | - |
flower | - |
Saussurea medusa | - |
flower | expression in flowers is about 8fold higher compared with leaves and roots | Saussurea medusa | - |
leaf | - |
Saussurea medusa | - |
leaf | expression in flowers is about 8fold higher compared with leaves and roots | Saussurea medusa | - |
additional information | expression level of SmDFR is higher in flowers compared with both leaves and roots | Saussurea medusa | - |
root | - |
Saussurea medusa | - |
root | expression in flowers is about 8fold higher compared with leaves and roots | Saussurea medusa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-dihydrokaempferol + NADPH + H+ | - |
Saussurea medusa | cis-3,4-leucopelargonidin + NADP+ | - |
? | |
(+)-dihydroquercetin + NADPH + H+ | - |
Saussurea medusa | cis-3,4-leucocyanidin + NADP+ | - |
? | |
dihydroquercetin + NADPH + H+ | - |
Saussurea medusa | leucocyanidin + NADP+ | - |
? | |
additional information | the enzyme catalyzes the reduction of dihydroflavonols to leucoanthocyanins. But SmDFR also possesses flavanone 4-reductase (FNR) activity and can catalyze the conversion of eridictyol to luteoforol, EC 1.1.1.234 | Saussurea medusa | ? | - |
? | |
additional information | no activity in reduction of dihydromyricetin | Saussurea medusa | ? | - |
? | |
additional information | no substrate: dihydromyricetin. enzyme additionally shows flavanone-4-reductase activity, EC 1.1.1.234 | Saussurea medusa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 38600, SDS-PAGE | Saussurea medusa |
Synonyms | Comment | Organism |
---|---|---|
DFR | - |
Saussurea medusa |
dihydroflavonol 4-reductase | - |
Saussurea medusa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Saussurea medusa |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the plant DFR superfamily, phylogenetic analysis | Saussurea medusa |
metabolism | the DFR gene is a key gene late in the flavonoid biosynthesis pathway, overview. The enzyme is a key enzyme in the biosynthesis of anthocyanidins, proanthocyanidins, and other flavonoids, and also possesses flavanone 4-reductase activity | Saussurea medusa |
physiological function | the enzyme is of importance in plant development | Saussurea medusa |